MOSCOW/DONETSK, Feb 22 (Reuters) - The United States and its European allies are poised to announce harsh new sanctions against Russia on Tuesday after President Vladimir Putin formally recognised two breakaway regions in eastern Ukraine, escalating a security crisis on the continent.

The Ukrainian military said two soldiers were killed and 12 wounded in shelling by pro-Russian separatists in the east in the past 24 hours, the most casualties this year, as ceasefire violations increased.

Putin's announcement on Monday drew international condemnation and immediate U.S. sanctions, with President Joe Biden signing an executive order to halt U.S. business activity in the breakaway regions.

"The United States will impose sanctions on Russia for this clear violation of international law and Ukraine sovereignty and territorial integrity," Linda Thomas-Greenfield, U.S. ambassador to the United Nations, told reporters after an emergency meeting of the Security Council late on Monday.

"We can, will, and must stand united in our calls for Russia to withdraw its forces, return to the diplomatic table and work toward peace." read more

A British government minister said Russia's invasion of Ukraine was already under way, so Britain would sanction Russia.

"You can conclude that the invasion of Ukraine has begun," Health Secretary Sajid Javid said.

Britain said it had drawn up sanctions to target those complicit in the violation of Ukraine’s territorial integrity, and those measures would come into force on Tuesday.

France and Germany also agreed to respond with sanctions.

China said it was concerned about the worsening situation and called for all parties to exercise restraint while Japan said it was ready to join international sanctions on Moscow in the event of a full-scale invasion.

The Russian U.N. ambassador, Vassily Nebenzia, warned Western powers to "think twice" and not worsen the situation.

A Reuters witness saw tanks and other military hardware moving through the separatist-controlled city of Donetsk on Monday hours after Putin formally recognised the breakaway regions and ordered the deployment of Russian forces to "keep the peace". No insignia were visible on the vehicles.

Ukraine's military said on its Facebook page it had recorded 84 cases of shelling by separatists who it said had opened fire on about 40 settlements along the front line with heavy artillery, in breach of ceasefire agreements.

The Interfax news agency cited a separatist official as saying Ukrainian forces had killed three civilians.

Russia denies any plan to attack its neighbour, but it has amassed troops on Ukraine's borders and threatened "military-technical" action unless it receives sweeping security guarantees, including a promise that Ukraine will never join NATO.

A senior U.S. official said the deployment of Russian troops to the breakaway enclaves did not merit the harshest sanctions the United States and its allies had prepared in the event of a full-scale invasion, as Russia already had troops there.

GRIEVANCES

Russian-backed separatists in Ukraine's Donetsk and Luhansk regions - collectively known as the Donbass - broke away from Ukrainian government control in 2014 and proclaimed themselves independent "people's republics".

The Organization for Security and Co-operation in Europe called on Russia to the decision to recognise the regions saying it would only fuel tension and separate the populations living in the regions from the rest of their country, Ukraine.

Russia needed to ratify its friendship treaties with the regions before it could discuss matters like the exact borders of the territories, RIA news agency reported, citing the foreign ministry. Russia's parliament is expected to review friendship treaties on Tuesday.

Ukrainian President Volodymyr Zelenskiy, who received a call from Biden to express solidarity on Monday, accused Russia of wrecking peace talks and ruled out territorial concessions.

Rising fears of a major war in Europe pushed oil prices to a seven-year high on Tuesday, while safe-havens currencies like the yen rallied and global stocks tumbled. read more The rouble extended its losses as Putin spoke, at one point sliding beyond 80 per dollar. read more

In a lengthy televised address on Monday packed with grievances against the West, a visibly angry Putin said eastern Ukraine was ancient Russian land. read more .

Putin delved into history as far back as the Ottoman empire and expressed frustration that Russia's demands for a rewriting of Europe's security arrangements had been repeatedly rebuffed. read more

"I deem it necessary to make a decision that should have been made a long time ago - to immediately recognise the independence and sovereignty of the Donetsk People's Republic and the Lugansk People's Republic," Putin said.

Putin has for years worked to restore Russia's influence over nations that emerged after the collapse of the Soviet Union, with Ukraine holding an important place in his ambitions. Russia annexed Crimea from Ukraine in 2014.

A French presidential official said the speech "mixed various considerations of a rigid and paranoid nature".